An improved method of mitochondrial DNA isolation for XL-PCR

时多; 朱克军; 王学敏; 汪振诚; 郑建明; 缪明永; 焦炳华

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An improved method of mitochondrial DNA isolation for XL-PCR

Updated：2026-03-12

- An improved method of mitochondrial DNA isolation for XL-PCR
- An improved method of mitochondrial DNA isolation for XL-PCR
- 解放军医学杂志（英文版） 2006年第2期页码：82-85
- Affiliations：
  
  1. Department of Biochemistry and Molecular biology Second Military Medical University
  2. ,China
  3. Department of Pathology
  4. Changhai Hospital
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China （No. C30171030, C30472175）China Postdoctoral Science Foundation （No. 2004036014）
- DOI：
  中图分类号： R346
- 纸质出版：2006
- Accepted：
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An improved method of mitochondrial DNA isolation for XL-PCR[J]. 解放军医学杂志（英文版）, 2006,(2):82-85.

[1]时多,朱克军,王学敏,汪振诚,郑建明,缪明永,焦炳华.An improved method of mitochondrial DNA isolation for XL-PCR[J].Journal of Medical Colleges of PLA,2006(02):82-85.
An improved method of mitochondrial DNA isolation for XL-PCR[J]. 解放军医学杂志（英文版）, 2006,(2):82-85. DOI：

[1]时多,朱克军,王学敏,汪振诚,郑建明,缪明永,焦炳华.An improved method of mitochondrial DNA isolation for XL-PCR[J].Journal of Medical Colleges of PLA,2006(02):82-85. DOI：

摘要

Abstract

<正>Objective: To obtain high quality of mitochondrial DNA （mtDNA） and carry out extra-long PCR （XL-PCR）. Methods: Mitochondria were isolated by differential centrifugation

and membranes were disrupted using 10%SDS （pH 7. 0）. mtDNA was then extracted using phenol and chloroform. Results: The mtDNA obtained by using our improved method can be used as effective template for XL-PCR

and total mtDNA （16 kb） can be amplified easily. Conclusion: Our improved method is effective in preparing high quality of mtDNA

which can be used as template for XL-PCR.

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