<正>Objective:To explore the effects of predegenerated peripheral nerve graft （PPNG） combined with nerve growth factor （NGF） infusion on ascending sensory tract regeneration after spinal cord injury. Methods: Fifty female SD rats were randomly divided into 5 groups. Group A was treated with PPNG and NGF infusion

group B with PPNG

group C with NGF infusion

group D and group E were blank and normal control

respectively. Horseradish peroxidase-labled （HRP） tracing method was employed to evaluate the regeneration of injured nerves after 8 weeks. The extent of regeneration in and beyond the nerve graft was determined by counting the number of HRP-labeled fibers intersecting imaginary lines perpendicular to the axis of the graft and cord. For the sake of convenience

according to the relation of the PNG and spinal cord

6 model zones were divided

including caudal of spinal cord

caudal transition zone

caudal zone in graft

rostral zone in graft

rostral transition zone and rostral of spinal cord. Results: On the transverse section of caudal zone in graft

rostral zone in graft

rostral transition zone

the fibers in group A were significantly higher than that in group B and C （P<0. 05）. Conclusion: PPNG combined with NGF may significantly promote the regeneration of ascending long tract after spinal cord injury. The regenerative fibers can penetrate the 2 graft-host interface scars.