Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique

刘晓峰; 刘长江; 胡家露; 贾爱芹; 孙自勤

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Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique

Updated：2026-03-12

- Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique
- Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique
- 解放军医学杂志（英文版） 2006年第6期页码：365-368
- Affiliations：
  
  1. Department of Gastroenterology General Hospital of Jinan Military Area Command
  2. Institute of Gastroenterology Xijing Hospital Fourth Military Medical University
  3. ,Xi’an,710032
- Author bio：
- Funds：
- DOI：
  中图分类号： R392.1
- 纸质出版：2006
- Accepted：
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Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique[J]. 解放军医学杂志（英文版）, 2006,(6):365-368.

[1]刘晓峰,刘长江,胡家露,贾爱芹,孙自勤.Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique[J].Journal of Medical Colleges of PLA,2006(06):365-368.
Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique[J]. 解放军医学杂志（英文版）, 2006,(6):365-368. DOI：

[1]刘晓峰,刘长江,胡家露,贾爱芹,孙自勤.Immune responses in mice to oral administration of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit by a lavage technique[J].Journal of Medical Colleges of PLA,2006(06):365-368. DOI：

摘要

Abstract

<正>Objective: To determine whether attenuated Salmonella typhimurium producing Helicobacter pylori （Hp） urease subunit B（UreB）can elicit specific immune responses against Hp in mice tested by a lavage technique. Methods: Attenuated Salmonella typhimurium producing Hp UreB immunized orally Balb/c mice twice at a 3-week interval. After 12 weeks

mice intestinal secretions were obtained without harm by administering a lavage solution intragastrically. The mice intestinal secretions of immune group were also directly washed out after the mice were killed. The antibody responses were evaluated by using serum and intestinal fluid with ELISA assay. Results: The multiple oral immunizations with SL3261/ pTC01-UreB induced significantly Hp-specific mucosal IgA response as well as serum IgG response. The IgA was also consistently higher in the intestinal fluid obtained by the lavage solution than by direct washout. In addition

no obvious side effects and changes in gastric inflammation were observed in mice. Conclusion: The attenuated Salmonella typhimurium expressing Hp UreB may be used as an oral vaccine against Hp infection. And the lavage technique is an ideal method in the study of mucosal immune responses.

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