Screening of FOXP3-interacted proteins by yeast two-hybrid technique

Zhou Lina Wu Jun Luo Gaoxing He Weifeng Chen Xiwei Bo Ganping Yuan Shunzong Zhang Xiaorong Hu Xiaohong State Key Laboratory of Trauma; Burns and Combined Injury; Institute of Burns; Southwest Hospital; Third Military Medical University; Chongqing 400038; China

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Screening of FOXP3-interacted proteins by yeast two-hybrid technique

Updated：2026-03-12

- Screening of FOXP3-interacted proteins by yeast two-hybrid technique
- Screening of FOXP3-interacted proteins by yeast two-hybrid technique
- 解放军医学杂志（英文版） 2008年第2期页码：81-87
- Affiliations：
- Author bio：
- Funds：
  
  the National Natural Science Foundation of China（30500447）
- DOI：
  中图分类号： R151
- 纸质出版：2008
- Accepted：
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Screening of FOXP3-interacted proteins by yeast two-hybrid technique[J]. 解放军医学杂志（英文版）, 2008,(2):81-87.

[1].Screening of FOXP3-interacted proteins by yeast two-hybrid technique[J].Journal of Medical Colleges of PLA,2008(02):81-87.
Screening of FOXP3-interacted proteins by yeast two-hybrid technique[J]. 解放军医学杂志（英文版）, 2008,(2):81-87. DOI：

[1].Screening of FOXP3-interacted proteins by yeast two-hybrid technique[J].Journal of Medical Colleges of PLA,2008(02):81-87. DOI：

摘要

Abstract

<正>Objective:To screen the proteins interacting with the Treg specification factor forkhead box protein P3 （FOXP3） by yeast two-hybrid system.Methods:Human FOXP3 gene was amplified by nest RT-PCR from peripheral blood mononuclear cells （PBMC） and inserted into plasmid pGBKT7 to construct the bait vector

then the self-activation and toxicity of the bait vector in host yeast strain AH109 were observed.Thereafter

a human liver cDNA library was screened by the bait vector.The positive clones were selected out by nutrient-deficient culture and back-hybridizing.The sequences from the candidate positive clones were blasted and analyzed by bioinformatics methods.Results:The constructed bait vector encoding FOXP3 was found no self-activation and toxicity in yeast AH109.Three proteins which interacted with FOXP3

including tumor protein D52

splicing factor 3b subunit 1 and hypothetical protein

were identified.Conclusion:Three new candidate proteins interacting with FOXP3 are selected out by this yeast two-hybrid system and library

which may facilitate the further study of FOXP3 in Treg.

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