Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line

Li Jin Song Bo Chen Zhiwen Urinary Surgery Research Institute; Southwest Hospital; Third Military Medical University; Chongqing 400038; China Zeng Yijun Zhou Huchuan Wei Quanfang Yang Jin Department of Biochemistry; College of Medicine; China

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Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line

Updated：2026-03-12

- Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line
- Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line
- 解放军医学杂志（英文版） 2008年第2期页码：76-80
- Affiliations：
- Author bio：
- Funds：
  
  the National Natural Science Foundation of China（30471958）;the Natural Science Foundation of Chongqing（No.2006BB5045）
- DOI：
  中图分类号： R346
- 纸质出版：2008
- Accepted：
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Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line[J]. 解放军医学杂志（英文版）, 2008,(2):76-80.

[1].Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line[J].Journal of Medical Colleges of PLA,2008(02):76-80.
Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line[J]. 解放军医学杂志（英文版）, 2008,(2):76-80. DOI：

[1].Transcription-coupled repair pathway in UVC-induced SupF gene mutation in Tet-on 293 cell line[J].Journal of Medical Colleges of PLA,2008(02):76-80. DOI：

摘要

Abstract

<正>Objective:To explore the role of transcription-coupled repair （TCR） pathway in the UVC-induced SupF gene mutation in Tet-on 293 cell line

we designed and constructed a Tet-responsive plasmid DNA pTCR-C1

and utilized this pTCR-C1 plasmid to obtain the mutation frequency of SupF reporter gene induced by UVC in Tet-on 293 cell line. Methods:SupF gene was cloned into a Tet-responsive plamid pBI-L

which include a bidirectional Tet-responsive promoter

and was named pTCR-C1.The pTCR-C1 plasmid was transfected into Tet-on 293 cell line

and the mutation frequency of SupF reporter gene was detected in the presence and absence of DOX.Results:The pTCR-C1 plasmid was identified with the methods of restriction digestion and DNA sequencing.The mutation frequency of SupF reporter gene in the presence of DOX was higher than in the absence of DOX.Conclusion:The TCR pathway takes part in the UVC-induced SupF gene mutation in Tet-on 293 cell line.

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