The development and optimization of ELISA for the determination of tetrodotoxin

周玉; 李岩松; 潘风光; 柳增善; 王哲

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The development and optimization of ELISA for the determination of tetrodotoxin

Updated：2026-03-12

- The development and optimization of ELISA for the determination of tetrodotoxin
- The development and optimization of ELISA for the determination of tetrodotoxin
- 解放军医学杂志（英文版） 2007年第6期页码：347-351
- Affiliations：
  
  1. Key Laboratory of Zoonoses Ministry of Education
  2. Institute of Zoonoses.Jilin University
  3. College of Light Industry and Economics & Management
  4. ,China
- Author bio：
- Funds：
  
  the grants from PhD Priming Foundation of Jilin University（430505010276）
- DOI：
  中图分类号： R114
- 纸质出版：2007
- Accepted：
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The development and optimization of ELISA for the determination of tetrodotoxin[J]. 解放军医学杂志（英文版）, 2007,(6):347-351.

[1]周玉,李岩松,潘风光,柳增善,王哲.The development and optimization of ELISA for the determination of tetrodotoxin[J].Journal of Medical Colleges of PLA,2007(06):347-351.
The development and optimization of ELISA for the determination of tetrodotoxin[J]. 解放军医学杂志（英文版）, 2007,(6):347-351. DOI：

[1]周玉,李岩松,潘风光,柳增善,王哲.The development and optimization of ELISA for the determination of tetrodotoxin[J].Journal of Medical Colleges of PLA,2007(06):347-351. DOI：

摘要

Abstract

<正>Objective:To optimize the ELISA for the determination of tetrodotoxin.Methods:A corn petitive enzyme-linked immunosorbent assay（ELISA） was used.In the ELISA.100μl antigen（1.0μg/ml） was coated on the microtiter plate for 60 min at 37℃or over night at 4℃.The plate was then washed 3 times with PBS-T for 3-5 s each time.The optimal incubation time for monoclonal antibody （mAb）

goat anti-mice IgG peroxidase conjugate and OPD were 30 min.20 rain and 10 min at 37℃

re- spectively.Results:The detection limit is 0.05 ng in each well.The curve was linear for TTX doses be tween 5 5 000 ng/ml （0.25 250 ng for every assay）.The linear regress equation was Y=0.30 88X-- 0.17 41 （R=0.99 01）.The average callback for TTX of muscles and gonads were 99.74% and 100.30%

respectively.The sensitivity of optimization ELISA was 5 times than traditional method and the time of 1.8 h were saved.Conclusion..The optimized ELISA is an ideal method for the determination of tetrodotoxin.

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