Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells

Wang Ding Miao Chunmu Gong Jianping Department of Hepatobiliary Surgery; Second Affiliated Hospital of Chongqing Medical University; Chongqing 400010; China

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Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells

Updated：2026-03-12

- Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells
- Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells
- 解放军医学杂志（英文版） 2008年第2期页码：70-75
- Affiliations：
- Author bio：
- Funds：
  
  the National Natural Science Foundation of China （30530360 and 30772098）
- DOI：
  中图分类号： R363
- 纸质出版：2008
- Accepted：
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Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells[J]. 解放军医学杂志（英文版）, 2008,(2):70-75.

[1].Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells[J].Journal of Medical Colleges of PLA,2008(02):70-75.
Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells[J]. 解放军医学杂志（英文版）, 2008,(2):70-75. DOI：

[1].Role of liver X receptors alpha agonist on expressions of LPS-induced inflammatory response associated factor IRAK-4 and NF-kappaB in Kupffer cells[J].Journal of Medical Colleges of PLA,2008(02):70-75. DOI：

摘要

Abstract

<正>Objective:To explore the role of activated liver X receptor a （LXRα） on the expressions of interleukin-1 receptor associated kinase-4 （IRAK-4） and NF-kappaB （NF-κB） in the inflammatory response which induced by LPS in the Kupffer cells and to investigate the possible mechanisms of LXRαnegative regulation of inflammatory response. Methods:The Kupffer cells were isolated from male Kunming mice by collagen perfusion in situ.And these cells were divided into 4 groups:normal control group

LPS treatment group

LXRαagonist T0901317 treatment group

LPS and T0901317 combined treatment group.The LPS treatment group were treated with a final concentration of 1μg/ml LPS in RPMI 1640 and cultured for 6 h

the T0901317 treatment group were treated with a final concentration of 5μg/ml in RPMI 1640 and cultured for 24 h

and the combined treatment group received pre-culture for 24 h with a final concentration of 1μg/ml T0901317 in RPMI 1640 and then cultured for 6 h with a final concentration of 5μg/ml LPS in RPM! 1640.All groups were cultured for 30 h.The expression of LXRα

IRAK-4 and NF-κB at mRNA and protein levels were detected by real-time PCR and Western blotting

and the TNF-αand IL-1βlevels were detected by ELISA. Results:The levels of LXRαmRNA and protein were highest in T0901317 group

and lowest in LPS group （P<0.05）. The level of IRAK4 and NF-κB mRNAs and proteins were evidently lower in the Combined-treated group than in LPS group （P<0.05）.And the level of TNF-αand IL-1 were observed highest in LPS group （P<0.05）

but no difference among the Control group

T0901317 group and Combined-treated group （P>0.05）.Conclusion:These date suggest that the LXR agonists can effectively up-regulate the expressions of LXRαmRNA and protein and inhibit the inflammatory response.This may be via down-regulating the expressions of IRAK4 and NF-κB at mRNA and protein levels.

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