Objective: To explore the impact of s-phase kinase-associated protein 2 （Skp2） on cervical cancer cell proliferation and the relationship between Skp2 and expression of cell regulation factors and transcription factors. Methods: RNAi technology was used to silence Skp2 gene in HeLa cells. After interference

RT-PCR was used for detection of Skp-2 mRNA

and Western blotting and flow cytometry were used for protein expression analysis. Results: siRNA significantly inhibited HeLa cell proliferation （P<0.05） and increased HeLa apoptosis

and G1/G0 phase cells were increased significantly （P<0.01）. Skp2 siRNA transfected HeLa cells effectively reduced Skp2 protein levels

while p27 and p-p53 protein levels were increased significantly. RT-PCR results showed that after interference Skp2 mRNA

c-myc mRNA and cyclin A mRNA expressions decreased significantly compared with those in control group （P<0.01）

and p27mRNA expression level was significantly higher （P<0.01）. Conclusion: The change of Skp2 expression affects the expression of the cell cycle protein

thus affecting proliferation and apoptosis of HeLa cells. Skp2 protein plays an important role in the progression of cervical cancer; yet the specific mechanism still needs further study.