TLR4 -2242 T→C variant increases transcriptional activity of its promoter

Wang Yongtang; Jiang Jianxin; Liu Qing; Duan Zhaoxia; Gu Wei; Zeng Ling; Chen Kehong State Key Laboratory of Trauma; Burns and Combined Injury; Institute of Surgery Research; Daping Hospital; Third Military Medical University; Chongqing 400042; China

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TLR4 -2242 T→C variant increases transcriptional activity of its promoter

Updated：2026-03-12

- TLR4 -2242 T→C variant increases transcriptional activity of its promoter
- TLR4 -2242 T→C variant increases transcriptional activity of its promoter
- 解放军医学杂志（英文版） 2009年24卷第2期页码：69-75
- Affiliations：
- Author bio：
- Funds：
  
  Supported by the Major State Basic Research Development Program of China （2005CB522602）;the National Funds for Outstanding Youth Scientists （30325040）
- DOI：
  中图分类号： R515.3
- 纸质出版：2009
- Accepted：
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TLR4 -2242 T→C variant increases transcriptional activity of its promoter[J]. 解放军医学杂志（英文版）, 2009,24(2):69-75.

[1].TLR4 -2242 T→C variant increases transcriptional activity of its promoter[J].Journal of Medical Colleges of PLA,2009,24(02):69-75.
TLR4 -2242 T→C variant increases transcriptional activity of its promoter[J]. 解放军医学杂志（英文版）, 2009,24(2):69-75. DOI：

[1].TLR4 -2242 T→C variant increases transcriptional activity of its promoter[J].Journal of Medical Colleges of PLA,2009,24(02):69-75. DOI：

摘要

Abstract

Objective:To investigate the effects of -2242

-1892 and -1837 single nucleotide polymorphisms（SNPs） on toll-like receptor 4（TLR4） promoter activity.Methods:Polymerase chain reaction（PCR） and site direct mutation technology were used to construct TLR4 basic promoter and -2242C

-1892A and -1837G mutate promoter plasmids.Dual-Luciferase Reporter assay system was used to detect the activity of constructed promoter following human embryonic kidney（HEK） 293 cells were transiently cotransfected with the constructed plasmids and the control plasmid pRL-CMV.Results:In HEK293 cells

the activity of -2242C mutate promoter was higher than -2242T promoter

and there was no significant difference when both -1892A and -1837G mutate promoter compared with -1892G and -1837A promoter

respectively.Conclusion:It is implied that -2242T→C base variation can enhance the activity of TLR4 promoter

while -1892 and -1837 SNPs have no effect on TLR4 promoter activity.

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