Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells

Ji Kaihong; Xiong Jun; Fan Lixing; Hu Kaimeng; Liu Houqi Research Center of Developmental Biology; Department of Histology and Embryology; College of Basic Medical Sciences; Second Military Medical University; Shanghai 200433; China

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Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells

Updated：2026-03-12

- Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells
- Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells
- 解放军医学杂志（英文版） 2009年24卷第2期页码：84-91
- Affiliations：
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China（30600651）;the Cooperation Foundation for Overseas Young Scientists （30428001）
- DOI：
  中图分类号： R751
- 纸质出版：2009
- Accepted：
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Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells[J]. 解放军医学杂志（英文版）, 2009,24(2):84-91.

[1].Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells[J].Journal of Medical Colleges of PLA,2009,24(02):84-91.
Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells[J]. 解放军医学杂志（英文版）, 2009,24(2):84-91. DOI：

[1].Ex vivo differentiation of multipotent adult progenitor cells to skin epidermal cells[J].Journal of Medical Colleges of PLA,2009,24(02):84-91. DOI：

摘要

Abstract

Objective:By establishing the indirect contact co-culture system

we studied the in vitro condition for MAPCs differentiating into epidermal cells and the transformation of MAPCs into epidermal cell phenotype.Methods:Cell culture insert membrane was used for substitute basal membrane and MAPCs

fibroblast cells（FCs） and mixture of MAPCs and epidermal cells and FCs were separately implanted into 2 sides of it.PKH26 was used to label cloned MAPCs;type IV collagen rapid adhering method was used to isolate and culture the skin epidermal cells from 1-day-old SD rat.Results:Part of the MAPCs transformed into cells expressing keratin in the presence of peripheral epithelia and FCs.Type IV collagen rapid adhering method successfully selected rats’ epidermal stem cells.The mixture of the 2 kinds of cells or indirect culture might promote the differentiation through mesenchymal factors secreted by dermis FC.Conclusion:We were the first to have established the in vitro model of MAPCs differentiation into epidermal cells

in which MAPCs were transformed into epithelium-like cells.

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