Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line

Zhou Changlin1; Zhou Feiguo2; Gao Chunfang3; Wang Hao4; Xu Lingling4; Zhang Lingzheng4; Chen Jie51Department of Healthcare; Changzheng Hospital; Second Military Medical University; Shanghai 200003; China 2Department of Liver Surgery; Eastern Hepatobiliary Hospital; Shanghai 200438; China 3Department of Laboratory Medicine; Eastern Hepatobiliary Hospital; China 4Department of Laboratory Medicine; Changzheng Hospital; Shanghai 200003; China 5Liver Fibrosis Research Center; Lifetech Group; Hongkong

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Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line

Updated：2026-03-12

- Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line
- Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line
- 解放军医学杂志（英文版） 2008年23卷第6期页码：336-345
- Affiliations：
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China （30270605）
- DOI：
  中图分类号： R735.7
- 纸质出版：2008
- Accepted：
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Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line[J]. 解放军医学杂志（英文版）, 2008,23(6):336-345.

[1].Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line[J].Journal of Medical Colleges of PLA,2008,23(06):336-345.
Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line[J]. 解放军医学杂志（英文版）, 2008,23(6):336-345. DOI：

[1].Biological effects of extract from newborn porcine liver on hepatocytes,hepatic stellate cells,and hepatoma cell line[J].Journal of Medical Colleges of PLA,2008,23(06):336-345. DOI：

摘要

Abstract

Objective: Porcine liver extract has been shown to be effective in the clinical treatment of severe hepatitis. The aim of the present study was to study its antifibrotic as well as immune regulatory effect in vitro. Methods: Hepatocytes

hepatic stellate cells （HSCs）

hepatoma cell line （HepG2） and human peripheral blood mononuclear cells （PMNCs） were studied with respect to proliferation

extracellular matrix production and apoptotic activities by proliferation assay

radioimmunoassay

gene transfection

reporter gene analysis and flow cytometry

respectively. Results: A strong stimulatory proliferation effect was observed in hepatocytes

and an inhibitory effect was found in HSCs. Hyaluronic acid （HA） production and reporter gene activities driven by various α1（I） procollagen gene promoters in HSC-T6 were significantly decreased after treatment with the extract. Fluo-Anexin V binding apoptotic HepG2 cells were more prominent in the presence of 60 μg/ml extract. More CD4+/CD69+ positive T lymphocytes existed in the presence of the extract. Conclusion: Porcine liver extract is effective for antifibrogenesis via hepatocyteregeneration

HSC and hepatoma cell inhibition in vitro. The elevation of active T lymphocytes is helpful for immune surveillance. Fine mapping of the extract is necessary in order to get definite molecules which are essential in all described functions.

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