Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers

Lu Jun1; Jiang Shan2; Ye Song1; Hu Zongchang3 1 Medical College; Anhui University of Science & Technology; Huainan 232001; China 2Department of Mining Engineering; Huainan Vocational & Technical College; Huainan 232001; China 3Affiliated Hospital of Anhui University of Science & Technology; Huainan 232001; China

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Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers

Updated：2026-03-12

- Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers
- Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers
- 解放军医学杂志（英文版） 2009年24卷第4期页码：223-227
- Affiliations：
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Universities of Anhui Province （KJ2008A152）;the Natural Science Foundation of the Committee of Education of Anhui Province （2005KJ238）
- DOI：
  中图分类号： R135.2
- 纸质出版：2009
- Accepted：
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Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers[J]. 解放军医学杂志（英文版）, 2009,24(4):223-227.

[1].Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers[J].Journal of Medical Colleges of PLA,2009,24(04):223-227.
Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers[J]. 解放军医学杂志（英文版）, 2009,24(4):223-227. DOI：

[1].Sequence analysis on drug-resistant rpoB gene of Mycobacterium tuberculosis L-form of isolated from pneumoconiosis workers[J].Journal of Medical Colleges of PLA,2009,24(04):223-227. DOI：

摘要

Abstract

Objective:To study the characteristics of drug-resistant genetic mutation of rpoB on coal workers’ pneumoconiosis complicated with L-form of Mycobacterium tuberculosis. Methods: A total of 42 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected

including 31 drug-resistant strains. Their genomes DNA were extracted

the target genes were amplified by PCR

and the hot regions in the rpoB gene were analyzed by automated DNA sequenator. Results: No mutation of rpoB gene was identified in 11 rifampicin-sensitive strains while conformation changes were found in 31 rifampicin-resistant strains. The mutation rate was 93.55% （29/31） in resistant strains

mainly concentrated in codon 531 （51.6%

16/31） and 526 （32.26%

10/31）. Base substitutions happened

including 27 unit point mutation and 2 two point mutation. The mutation of codon 516 that new found wasn’t reported by internal and overseas scholars. Conclusion: The substitution of highly conserved amino acids encoded by rpoB gene results in the molecular mechanism responsible for rifampicin resistance in Mycobacterium tuberculosis L-forms. It also proves that rpoB gene is diversiform.

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