Objective:To analyze the antifungal effects of Chinese herb monomers

i.e.berberine

baicalin

eugenol and curcumin

on Candida albicans.Methods:After Candida albicans strain Y01-09 was incubated for 48 h in YEPD broth which contained different concentrations of Chinese herb components

the cell cycle

fluorescent intensity and the size of cell volume were detected by flow cytometry.Results:The 4 Chinese herb monomers could affect the cell cycle of Candida albicans in different ranges.The ratio of cells in S-G2-M period decreased as the agents concentration increased

indicating that the cell division was inhibited.The fluorescent intensity of Candida albicans cells became weaker after being incubated

which reflected the loss of DNA fragments.The higher the concentration was

the weaker the fluorescent intensity became.The cell size

cell diopter and particle size changed much as the agents concentration increased.Conclusion:Chinese herb monomers play the antifungal role in inhibiting cell division.FCM could be used to determine the susceptibility of antifungal agents.