Isolation and rapid genetic characterization of a novel T4-like bacteriophage

Wang Sheng~(1Δ); Jiang Huanhuan~(1Δ); Chen Jiankui~(2); Liu Dabin~1; Li Cun~1; Pan Bo~1; An Xiaoping~1; Zhang Xin~1; Zhou Yusen~1; Tong Yigang~(1) 1 State Key Laboratory of Pathogen and Biosecurity; Beijing Institute of Microbiology and Epidemiology; Beijing 100071; China 2 Clinical Laboratory; No.307 Hospitl of PLA; Beijing 100071; China

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Isolation and rapid genetic characterization of a novel T4-like bacteriophage

Updated：2026-03-12

- Isolation and rapid genetic characterization of a novel T4-like bacteriophage
- Isolation and rapid genetic characterization of a novel T4-like bacteriophage
- 解放军医学杂志（英文版） 2010年25卷第6期页码：331-340
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  中图分类号： Q939.4
- 纸质出版：2010
- Accepted：
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Isolation and rapid genetic characterization of a novel T4-like bacteriophage[J]. 解放军医学杂志（英文版）, 2010,25(6):331-340.

[1].Isolation and rapid genetic characterization of a novel T4-like bacteriophage[J].Journal of Medical Colleges of PLA,2010,25(06):331-340.
Isolation and rapid genetic characterization of a novel T4-like bacteriophage[J]. 解放军医学杂志（英文版）, 2010,25(6):331-340. DOI：

[1].Isolation and rapid genetic characterization of a novel T4-like bacteriophage[J].Journal of Medical Colleges of PLA,2010,25(06):331-340. DOI：

摘要

Abstract

<正>Ubiquitous on earth

bacteriophages are the most abundant entities in every ecosystem

but human knowledge about them is still limited compared with that about other forms of organisms.To enrich human knowledge and promote the utilization of bacteriophages

it is necessary to isolate and characterize as many as possible different bacteriophages.Here we describe the isolation of a T4-like bacteriophage IME08 and a rapid method for its genetic characterization.With this method we easily cloned a few random fragments of the bacteriophage genome.Sequence analysis of these random clones showed that bacteriophage IME08 shared the highest sequence similarity with T4-like Enterobacteria phage T4（94%identity）

JS98（95% identity）

JS10（95%identity） and RB14（94%identity） respectively

which suggested that IME08 belonged to T4-like bacteriophage genus.

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