Objective: To detect the expressions of brain-derived neurotrophic factor（BDNF） and nerve growth factor（NGF） in purified rat choroid plexus epithelial cells in vitro. Methods: Primary and passage choroid plexus epithelial cells were obtained from newborn

one-day Sprague-Dawley rats. The expressions of BDNF and NGF were measured by qRT-PCR and Western blottingting. The secretions of BDNF and NGF were detected by ELISA. Cell supernatants of primary cells

purified cells and passage 1 cells were harvested. Results: The expression of BDNF in the purified cells was significantly lower than that in the primary cells（P<0.05）

and it in the primary cells and the purified cells was significantly higher than that in the passage 1 cells（P<0.05）. The expression of NGF was significantly higher in the purified cells than in the primary cells and the passage 1 cells（P<0.05）. It in the passage 1 cells was significantly higher than that in the primary cells（P<0.05）. Conclusion: The time of CPECs transplantation for central nervous system diseases should be selected based on their secretory function and features

which could lead to better and more effective treatment.