Applications of CRISPR-Cas9 mediated genome engineering
Applications of CRISPR-Cas9 mediated genome engineering
- MMR 2015年2卷第1期 页码：11-17
- Affiliations：
State Key Laboratory of Proteomics, Collaborative Innovation Center for Cardiovascular Disorders, Genetic Laboratory of Development and Disease, Institute of Biotechnology, Beijing 100071, China
- Author bio：
*: yangx@bmi.ac.cn
- Funds：
DOI：
中图分类号：-
纸质出版：2015-03
- Accepted：
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Applications of CRISPR-Cas9 mediated genome engineering[J]. MMR, 2015,2(1):11-17.
Xiao Yang. Applications of CRISPR-Cas9 mediated genome engineering[J]. Military Medical Research, 2015, 2(1): 11-17.
摘要
Abstract
Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development
normal physiology
and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases
such as clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9
is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models
somatic genome engineering
and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine
ranging from basic research to human gene therapy.
关键词
Keywords
references
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