A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing
COMMENTARY|Updated:2022-12-27
|
A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing
A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing
MMR2015年2卷第3期 页码:121-122
Affiliations:
State Key Laboratory of Molecular Oncology, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 200021, China
Author bio:
*: qianhaili001@163.com
Funds:
DOI:
中图分类号:
纸质出版:2015-09
Accepted:
Scan QR Code
A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing[J]. MMR, 2015,2(3):121-122.
Chun-Xiao Li, Hai-Li Qian. A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing[J]. Military Medical Research, 2015, 2(3): 121-122.
A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing[J]. MMR, 2015,2(3):121-122.DOI:
Chun-Xiao Li, Hai-Li Qian. A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing[J]. Military Medical Research, 2015, 2(3): 121-122.DOI:
A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing
professor Xiao Yang authored a review on the development of CRISPR-Cas9 techniques in the journal of
Military Medical Research
. This review provided a valuable overview of this major scientific advance. It has been four years since the first publication of the CRISPR-Cas9 breakthrough. The use of this technique has expanded into various scientific areas and is being developed into a systematic technical platform that may contribute to many bioengineering fields involving DNA sequence editing.
关键词
Keywords
references
Yang X . Applications of CRISPR-Cas9 mediated genome engineering . Mil Med Res . 2015 ; 2 : 11 .
Jinek M , Chylinski K , Fonfara I , Hauer M , Doudna JA , Charpentier E . A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity . Science . 2012 ; 337 : 816 - 21 .
Doudna JA , Charpentier E . Genome editing. The new frontier of genome engineering with CRISPR-Cas9 . Science . 2014 ; 346 : 1258096 .
Ran FA , Hsu PD , Wright J , Agarwala V , Scott DA , Zhang F . Genome engineering using the CRISPR-Cas9 system . Nat Protoc . 2013 ; 8 : 2281 - 308 .
Shalem O , Sanjana NE , Hartenian E , Shi X , Scott DA , Mikkelsen TS , et al . Genome-scale CRISPR-Cas9 knockout screening in human cells . Science . 2014 ; 343 : 84 - 7 .
Guo Y , Xu Q , Canzio D , Shou J , Li J , Gorkin DU , et al . CRISPR Inversion of CTCF Sites Alters Genome Topology and Enhancer/Promoter Function . Cell . 2015 ; 162 : 900 - 10 .
Kleinstiver BP , Prew MS , Tsai SQ , Topkar VV , Nguyen NT , Zheng Z , et al . Engineered CRISPR-Cas9 nucleases with altered PAM specificities . Nature . 2015 ; 523 : 481 - 5 .
Samai P , Pyenson N , Jiang W , Goldberg GW , Hatoum-Aslan A , Marraffini LA . Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity . Cell . 2015 ; 161 : 1164 - 74 .
Maruyama T , Dougan SK , Truttmann MC , Bilate AM , Ingram JR , Ploegh HL . Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining . Nat Biotechnol . 2015 ; 33 : 538 - 42 .
Kim D , Bae S , Park J , Kim E , Kim S , Yu HR , et al . Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells . Nat Methods . 2015 ; 12 : 237 - 43 .
Liang P , Xu Y , Zhang X , Ding C , Huang R , Zhang Z , et al . CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes . Protein Cell . 2015 ; 6 : 363 - 72 .
Lanphier E , Urnov F , Haecker SE , Werner M , Smolenski J . Don't edit the human germ line . Nature . 2015 ; 519 : 410 - 1 .
Applications of CRISPR-Cas9 mediated genome engineering
地铁高架槽形梁足尺模型破坏试验与空间分析研究
轨道电路多音调频信号的数字解调方法
二取二制式计算机联锁系统中的通信技术
架悬机车驱动装置悬挂参数及结构的研究
相关作者
Xiao Yang
相关机构
State Key Laboratory of Proteomics, Collaborative Innovation Center for Cardiovascular Disorders, Genetic Laboratory of Development and Disease, Institute of Biotechnology