The Construction fo Hybrid Plasmid pBR322:RSF1010

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The Construction fo Hybrid Plasmid pBR322:RSF1010

Updated：2026-03-12

- The Construction fo Hybrid Plasmid pBR322:RSF1010
- The Construction fo Hybrid Plasmid pBR322:RSF1010
- 解放军医学杂志（英文版） 1986年第1期页码：6-12
- Affiliations：
  
  1. Laboratory of Molecular Genetics Second Military Medical College
  2. ,Shanghai
- Author bio：
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  中图分类号：
- 纸质出版：1986
- Accepted：
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The Construction fo Hybrid Plasmid pBR322:RSF1010[J]. 解放军医学杂志（英文版）, 1986,(1):6-12.

[1]温肇荣.The Construction fo Hybrid Plasmid pBR322:RSF1010[J].Journal of Medical Colleges of PLA,1986(01):6-12.
The Construction fo Hybrid Plasmid pBR322:RSF1010[J]. 解放军医学杂志（英文版）, 1986,(1):6-12. DOI：

[1]温肇荣.The Construction fo Hybrid Plasmid pBR322:RSF1010[J].Journal of Medical Colleges of PLA,1986(01):6-12. DOI：

摘要

Abstract

<正> The plasmids pBR322 and RSF1010

extracted from E.coli HB802 and E.coliC600 respectively

were treated with restrictive endonuclease EcoRI andligated with T4 DNA Iigase.C600 strains were then transformed with thehybrid plasmid（pSMMl）

and thus253transformants were obtained.Thefigures of gel electrophoresis and electromicrograph proved that pSMMlwas a pBR322:RSF1010 composite hybrid.In E.coli C600 pSMMl obtainedresistance against ampieilline（Ap）

tetracycline（Tc）and streptomycin（Sm）.The orientation of pBR322 and RSF1010 in hybrid pSMMl and the failureof introduction of this hybrid into P.putida AC10 were discussed.

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