Latest Issue

Volume 0 ， Issue 2 ， 1998

A study of the relationship between frequency of allelic deletion of chromosome 17p and late-stage colorectal carcinoma Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 79-82(1998)

Keywords：late stage colorectal carcinomas;allelic deletion;P53 gene

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<Citation> <Bulk Citation> 32970857 false

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Coexistence of nitric oxide synthase with γ-aminobutyric acid during seizure induced by kainic acid in SD rats Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 83-87(1998)

Keywords：nitric oxide (NO);nitric oxide synthase (NOS);seizures;kainic acid (KA);γ-amino-butyricacid (GABA);NADPH diaphorase (Nd)

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Establishment of transgenic mouse lineages containing copies of an integrated pSPORT1 plasmid Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 88-92(1998)

Keywords：transgenic mice;animals;mutagenesis;lacI gene;plasmid

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<Citation> <Bulk Citation> 32971099 false

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Detection of hepatocellular carcinoma cells in the peripheral blood with reverse--transcription polymerase chain reaction Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 93-96(1998)

Keywords：hepatocellular carcinoma;circulating cells;alpha-fetoprotein;reverse transcription-polymerase chain reaction;mRNA

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<Citation> <Bulk Citation> 32971028 false

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Changes of membrane-bound and -secreted TNF in rat Kupffer cell after thermal injury Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 97-101(1998)

Keywords：tumor necrosis factor;Kupffer cells;burn;flow cytometry

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<Citation> <Bulk Citation> 32971641 false

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Hepatitis G virus: a full-length cDNA clone Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 101(1998)

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<Citation> <Bulk Citation> 32971803 false

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Transsphenoidal microsurgical operation for pituitary adenomas with suprasellar extensions Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 102-105(1998)

Keywords：pituitary adenoma;suprasellar extension;transsphenoidal surgery, microsurgery

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<Citation> <Bulk Citation> 32971746 false

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Construction and application of gene targeting replacement vector for mouse coagulation factor IX gene Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 106-110(1998)

Keywords：gene targeting;embryonic stem cells;replacement vector;hemophilia B;coagulation factor IX gene

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<Citation> <Bulk Citation> 32971309 false

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Expression and significance of IL-6 mRNA and its protein in liver after injury in rats Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 111-114(1998)

Keywords：burns;endotoxemia;interleukin-6;immunohistochemistry;in situ hybridization;rat

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<Citation> <Bulk Citation> 32970809 false

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Endovascular embolization of cerebral arteriovenous malformation with N-butyl-2 cyanoacrylate Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 115-117(1998)

Keywords：cerebral arteriovenous malformation;embolization;N-butyl-2-cyanoacrylate

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<Citation> <Bulk Citation> 32971532 false

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Role of transforming growth factor P and bone morphogenetic protein composite on repair of bone defects Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 118-122(1998)

Keywords：TGF-β;BMP;bone defect;bone repair;osteogenesis

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<Citation> <Bulk Citation> 32970974 false

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Sequence differences of rDNA-ITS2 and species-diagnostic PCR assay of Anopheles sinensis and Anopheles anthropophagus from China Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 123-127(1998)

Keywords：Anopheles sinensis;Anopheles anthropophagus;rDNA;internal transcribed spacer 2;PCR

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<Citation> <Bulk Citation> 32971247 false

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A new artificial composite allograft for burn wound coverage: Its manufacture, characteristics and clinical application Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 129-132(1998)

Keywords：collagen membrane;epidermal cell;fibroblast

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<Citation> <Bulk Citation> 32970751 false

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Tubal catheterization for transvaginal gamete intrafallopian transfer: an alterative technique for non-tubal infertility Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 133-135(1998)

Keywords：transvaginal GIFT;Infertility

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<Citation> <Bulk Citation> 32971590 false

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Distribution of SP receptor in the auditory centre of brainstem and in the medial geniculate body of guinea pigs Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 136-139(1998)

Keywords：substance P receptor;immunohistochemistry;brainstem;auditory centre;guinea pig

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Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining Cover Article Back Cover Article Enhanced Publication AI Introduction

卫立辛, 闫振林, 沈锋, 谢天培, 钱其军, 崔贞福, 郭亚军, 吴孟超
Issue 2, Pages: 140-142(1998)

Abstract：Telomerase is a ribonucleoprotein enzyme, which synthesizes telomeric repeats （TTAGGG） n.While germline cells and most malignant tumor cells express telomerase activity, normal somatic cells aregenerally deficient in telomerase activity. Our objective was to detect telomerase activity of human cells bysilver staining with telorneric repeat amplification protocol （TRAP） which is easy and quick. Comparing withradioisotopic TRAP, we examined the telomerase activity in telomerase-positive 293-cell and RNase-pretreated and heat-pretreated negative controls by silver staining TRAP. We detected telomerase activity in 2 strainsof human liver tumor cells （QGY7701 and SMMC7721 ）. The 293 cells （only 10 cells） and the 2 strains ofhuman liver tumor cells were all positive. while telomerase activity was not detected in the negative controls.These data suggest that non-radioisotopic silver staining TRAP is a specific, sensitive and fast assay fortelomerase activity. It was verified that the 2 strains of human liver tumor cells express telomerase activity.

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Protective effects of bactericidal/permeability-increasing protein against hemorrhagic shock and its mechanism Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 143-145(1998)

Keywords：hernorrhagic/shock;bactericidal/permeability-increa protein;endotoxin;tumor necrosis factor α;interleukin-6;rat

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<Citation> <Bulk Citation> 32971193 false

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Effect of nephrectomy on vascular renin gene expression and biosynthesis of vascular aldosterone Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 146-149(1998)

Keywords：:vascular renin;vascular aldosterone: nephrectomy

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<Citation> <Bulk Citation> 32971490 false

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Immunohistochemical localization of nitric oxide synthase in gastrointestinal tract of rats Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 150-152(1998)

Keywords：nitric oxide synthase;immunohistochemistry;gastrointestinal tract;rat

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<Citation> <Bulk Citation> 32971374 false

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Treatment of fatal coronary artery spasm by stent implantation: A case report Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 153-156(1998)

Keywords：variant;angina pectoris;percutaneous transluminal coronary angioplasty (PTCA);stent, intracoronary

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A Brief Account of Professor Zhong Shizhen Cover Article Back Cover Article Enhanced Publication AI Introduction

Vol. 0, Issue 2, Pages: 157(1998)

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Latest Issue

A study of the relationship between frequency of allelic deletion of chromosome 17p and late-stage colorectal carcinoma Cover Article Back Cover Article Enhanced Publication AI Introduction

Coexistence of nitric oxide synthase with γ-aminobutyric acid during seizure induced by kainic acid in SD rats Cover Article Back Cover Article Enhanced Publication AI Introduction

Establishment of transgenic mouse lineages containing copies of an integrated pSPORT1 plasmid Cover Article Back Cover Article Enhanced Publication AI Introduction

Detection of hepatocellular carcinoma cells in the peripheral blood with reverse--transcription polymerase chain reaction Cover Article Back Cover Article Enhanced Publication AI Introduction

Changes of membrane-bound and -secreted TNF in rat Kupffer cell after thermal injury Cover Article Back Cover Article Enhanced Publication AI Introduction

Hepatitis G virus: a full-length cDNA clone Cover Article Back Cover Article Enhanced Publication AI Introduction

Transsphenoidal microsurgical operation for pituitary adenomas with suprasellar extensions Cover Article Back Cover Article Enhanced Publication AI Introduction

Construction and application of gene targeting replacement vector for mouse coagulation factor IX gene Cover Article Back Cover Article Enhanced Publication AI Introduction

Expression and significance of IL-6 mRNA and its protein in liver after injury in rats Cover Article Back Cover Article Enhanced Publication AI Introduction

Endovascular embolization of cerebral arteriovenous malformation with N-butyl-2 cyanoacrylate Cover Article Back Cover Article Enhanced Publication AI Introduction

Role of transforming growth factor P and bone morphogenetic protein composite on repair of bone defects Cover Article Back Cover Article Enhanced Publication AI Introduction

Sequence differences of rDNA-ITS2 and species-diagnostic PCR assay of Anopheles sinensis and Anopheles anthropophagus from China Cover Article Back Cover Article Enhanced Publication AI Introduction

A new artificial composite allograft for burn wound coverage: Its manufacture, characteristics and clinical application Cover Article Back Cover Article Enhanced Publication AI Introduction

Tubal catheterization for transvaginal gamete intrafallopian transfer: an alterative technique for non-tubal infertility Cover Article Back Cover Article Enhanced Publication AI Introduction

Distribution of SP receptor in the auditory centre of brainstem and in the medial geniculate body of guinea pigs Cover Article Back Cover Article Enhanced Publication AI Introduction

Detection of telomerase activity in human cells with telomeric repeat amplification protocol by silver staining Cover Article Back Cover Article Enhanced Publication AI Introduction

Protective effects of bactericidal/permeability-increasing protein against hemorrhagic shock and its mechanism Cover Article Back Cover Article Enhanced Publication AI Introduction

Effect of nephrectomy on vascular renin gene expression and biosynthesis of vascular aldosterone Cover Article Back Cover Article Enhanced Publication AI Introduction

Immunohistochemical localization of nitric oxide synthase in gastrointestinal tract of rats Cover Article Back Cover Article Enhanced Publication AI Introduction

Treatment of fatal coronary artery spasm by stent implantation: A case report Cover Article Back Cover Article Enhanced Publication AI Introduction

A Brief Account of Professor Zhong Shizhen Cover Article Back Cover Article Enhanced Publication AI Introduction