Microfluidics-based strategies for molecular diagnostics of infectious diseases

Xin Wang; Xian-Zhe Hong; Yi-Wei Li; Ying Li; Jie Wang; Peng Chen; Bi-Feng Liu

doi:10.1186/s40779-022-00374-3

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Microfluidics-based strategies for molecular diagnostics of infectious diseases

REVIEW | Updated：2023-03-20

- Microfluidics-based strategies for molecular diagnostics of infectious diseases
- Military Medical Research Vol. 9, Issue 6, Pages: 727-753(2022)
- Affiliations：
  
  1.The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
  2.State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences - Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430071, China
  3.Department of Radiology, Canary Center at Stanford for Cancer Early Detection, School of Medicine, Stanford University, Palo Alto, CA 94304, USA
- Author bio：
  
  * gwchenpeng@hust.edu.cn;
  bfliu@mail.hust.edu.cn
- Funds：
- DOI：10.1186/s40779-022-00374-3
  CLC：
- Published：2022-12
- Accepted：
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Wang X, Hong XZ, Li YW, Li Y, Wang J, Chen P, et al. Microfluidics-based strategies for molecular diagnostics of infectious diseases. Mil Med Res. 2022;9(1):11.
DOI：

Wang X, Hong XZ, Li YW, Li Y, Wang J, Chen P, et al. Microfluidics-based strategies for molecular diagnostics of infectious diseases. Mil Med Res. 2022;9(1):11. DOI： 10.1186/s40779-022-00374-3.

摘要

Abstract

Traditional diagnostic strategies for infectious disease detection require benchtop instruments that are inappropriate for point-of-care testing (POCT). Emerging microfluidics

a highly miniaturized

automatic

and integrated technology

are a potential substitute for traditional methods in performing rapid

low-cost

accurate

and on-site diagnoses. Molecular diagnostics are widely used in microfluidic devices as the most effective approaches for pathogen detection. This review summarizes the latest advances in microfluidics-based molecular diagnostics for infectious diseases from academic perspectives and industrial outlooks. First

we introduce the typical on-chip nucleic acid processes

including sample preprocessing

amplification

and signal read-out. Then

four categories of microfluidic platforms are compared with respect to features

merits

and demerits. We further discuss application of the digital assay in absolute nucleic acid quantification. Both the classic and recent microfluidics-based commercial molecular diagnostic devices are summarized as proof of the current market status. Finally

we propose future directions for microfluidics-based infectious disease diagnosis.

关键词

Keywords

references

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