Potential roles of vitamin D binding protein in attenuating liver injury in sepsis

Kun Xiao; Du-Chao Zhang; Ye Hu; Li-Cheng Song; Jian-Qiao Xu; Wan-Xue He; Pan Pan; Yu-Wei Wang; Li-Xin Xie

doi:10.1186/s40779-022-00365-4

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Potential roles of vitamin D binding protein in attenuating liver injury in sepsis

RESEARCH | Updated：2023-02-28

- Potential roles of vitamin D binding protein in attenuating liver injury in sepsis
- Military Medical Research Vol. 9, Issue 5, Pages: 543-552(2022)
- Affiliations：
  
  1.Center of Pulmonary and Critical Care Medicine, Chinese People’s Liberation Army (PLA) General Hospital, No. 28, Fuxing Street, Haidian District, Beijing 100853, China
  2.Department of Pulmonary and Critical Care Medicine, Beijing Shijitan Hospital, Beijing 100071, China
  3.Medical School of Chinese People’s Liberation Army (PLA), Chinese PLA General Hospital, Beijing 100853, China
  4.Department of Geriatric Comprehensive Surgery, the Second Medical Center and National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, No. 28, Fuxing Street, Haidian District, Beijing 100853, China
- Author bio：
  
  * wyw2657@126.com;
  xielx301@126.com
- Funds：
- DOI：10.1186/s40779-022-00365-4
  CLC：
- Published：2022-10
- Accepted：
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Xiao K, Zhang DC, Hu Y, Song LC, Xu JQ, He WX, et al. Potential roles of vitamin D binding protein in attenuating liver injury in sepsis. Mil Med Res. 2022;9(1):4.
DOI：

Xiao K, Zhang DC, Hu Y, Song LC, Xu JQ, He WX, et al. Potential roles of vitamin D binding protein in attenuating liver injury in sepsis. Mil Med Res. 2022;9(1):4. DOI： 10.1186/s40779-022-00365-4.

摘要

Abstract

Background:

In sepsis

vitamin D binding protein (VDBP) has been shown to be low-expressed. The current study examined the relationship between serum VDBP level and liver injury in sepsis patients

as well as in a mouse model for sepsis and in cultured liver epithelial cell line exposed to lipopolysaccharide (LPS).

Methods:

The human study included 78 sepsis patients and 50 healthy volunteers. Sepsis patients were categorized into sepsis survivor group (

=43) and sepsis non-survivor group (

=35) based on 28-day mortality for data analysis. Adult male C57BL/6 mice were subjected to cecal ligation and puncture (CLP). Serum samples were collected on days 1

5 and 7 to determine the levels of VDBP

25-hydroxyvitamin D [25(OH)D

]

25-dihydroxyvitamin D [1

25(OH)

]

interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α). Potential protective effects of VDBP overexpression against LPS-induced liver damage were examined in cultured THLE2 cells.

Results:

Serum levels of VDBP

25(OH)D

and 1

25(OH)

were significantly lower in sepsis patients

. the healthy control (

0.001)

as well as in the sepsis non-survivor group

. the sepsis survivor group (

0.001

=0.0338

=0.0013

respectively). Lower serum VDBP level was associated with higher Acute Physiology and Chronic Health Evaluation (APACHE) II score (r=–0.2565

=0.0234) and Sequential Organ Failure Assessment (SOFA) score (r=–0.3522

=0.0016)

but lower serum albumin (ALB

=0.4628

0.001) and total protein (TP

=0.263

=0.02). In CLP mice

there was a 5-day period of serum VDBP reduction

followed by return towards the baseline on day 7. VDBP was also decreased in LPS-treated THLE2 cells (

0.001). VDBP overexpression reduced LPS-induced THLE2 damage. Reduced damage was associated with decreased oxidative stress and inactivation of the c-Jun N-terminal kinase signaling pathway.

Conclusions:

VDBP may be protective against sepsis-induced liver injury.

关键词

Keywords

references

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