Autologous omentum transposition for regeneration of a renal injury model in rats

Tayfun Bilgiç; Ümit İnce; Fehmi Narter

doi:10.1186/s40779-021-00361-0

Your Location：

Home >

Browse articles >

Autologous omentum transposition for regeneration of a renal injury model in rats

RESEARCH | Updated：2023-02-28

- Autologous omentum transposition for regeneration of a renal injury model in rats
- Military Medical Research Vol. 9, Issue 5, Pages: 534-542(2022)
- Affiliations：
  
  1.Acıbadem Kadıkoy Hospital of General Surgery, Istanbul 34718, Turkey
  2.Department of Pathology, Acıbadem Mehmet ali Aydınlar University, Istanbul 34684, Turkey
  3.Department of Urology, Acıbadem Mehmet ali Aydınlar University, Istanbul 34684, Turkey
- Author bio：
  
  * tbilgic77@gmail.com
- Funds：
- DOI：10.1186/s40779-021-00361-0
  CLC：
- Published：2022-10
- Accepted：
Scan QR Code
Bilgiç T, İnce Ü, Narter F. Autologous omentum transposition for regeneration of a renal injury model in rats. Mil Med Res. 2022;9(1):1.
DOI：

Bilgiç T, İnce Ü, Narter F. Autologous omentum transposition for regeneration of a renal injury model in rats. Mil Med Res. 2022;9(1):1. DOI： 10.1186/s40779-021-00361-0.

摘要

Abstract

Background:

After renal trauma

surgical treatment is vital

but sometimes there may be loss of function due to fibrosis. This study aimed to evaluate the effect of autologous omentum flaps on injured renal tissues in a rat model.

Methods:

A total of 30 Wistar albino rats were included and randomly divided equally into a control group and four intervention groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 group and primary repair 2 group) or transposition of the autologous omentum (omentum repair 1 group and omentum repair 2 group). Blood samples were taken preoperatively and on the 1st and 7th postoperative days in all groups and on the 18th postoperative day in the control and two intervention groups. All rats were sacrificed on the 7th or 18th day postoperatively

and their right kidneys were taken for histopathological evaluation.

Results:

The mean urea level significantly decreased from day 1 to day 7 and from day 1 to day 18 in the omentum repair 2 group (

=0.005 and

=0.004

respectively). There were no other significant changes in urea or creatinine levels within the intervention groups (P

0.05). There was no significant correlation between the urea and creatinine levels and the histological scores (P

0.05). The primary repair 1 and 2 groups had significantly higher median granulation and inflammation scores in the kidney specimen than the control and omentum repair groups (

0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (

0.05). The completion score for the healing process in the kidney specimen was significantly higher in the omentum repair groups than in the primary repair groups (

0.05). Granulation degree in the kidney specimen was strongly and positively correlated with the inflammation degree (

=0.824

0.001) and foreign body reaction in the kidney specimen (

=0.872

0.001)

and a strong and negative correlation with the healing process completion score in the kidney specimen (

=–0.627

=0.001). Inflammation degree in the kidney specimen was strongly and positively correlated with the foreign body reaction in the kidney specimen (

=0.731

=0.001)

and strongly and negatively correlated with the healing process completion score in the kidney specimen (

=–0.608

=0.002).

Conclusions:

Autologous omentum tissue for kidney injury repair attenuated inflammation and granulation. Additionally

the use of omental tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process with reduced fibrosis

tissue and function loss.

关键词

Keywords

references

Erlich T , Kitrey ND . Renal trauma: the current best practice . Ther Adv Urol . 2018 ; 10 ( 10 ): 295 - 303 .

Mann U , Zemp L , Rourke KF . Contemporary management of renal trauma in Canada: a 10-year experience at a level 1 trauma centre . J De L’association Des Urol Du Can . 2019 ; 13 ( 6 ): E177 - 82 .

Mansbridge MM , Ryan J , Hill DC , Wullschleger M . Renal trauma: a 3-year audit from a Gold Coast trauma centre . ANZ J Surg . 2019 ; 89 ( 4 ): 339 - 44 .

Opneja A , Kapoor S , Stavrou EX . Contribution of platelets, the coagulation and fibrinolytic systems to cutaneous wound healing . Thromb Res . 2019 ; 179 : 56 - 63 .

Nassar D , Letavernier E , Baud L , Aractingi S , Khosrotehrani K . Calpain activity is essential in skin wound healing and contributes to scar formation . PLoS One . 2012 ; 7 ( 5 ): e37084 .

Mao Y , Li H , Ding M , Hao X , Pan J , Tang M , et al . Comparative study of choke vessel reconstruction with single and multiple perforatorbased flaps on the murine back using delayed surgery . Ann Plast Surg . 2019 ; 82 ( 1 ): 93 - 8 .

Santucci RA , Wessells H , Bartsch G , Descotes J , Heyns CF , McAninch JW , et al . Evaluation and management of renal injuries: consensus statement of the renal trauma subcommittee . BJU Int . 2004 ; 93 ( 7 ): 937 - 54 .

Litbarg NO , Gudehithlu KP , Sethupathi P , Arruda JAL , Dunea G , Singh AK . Activated omentum becomes rich in factors that promote healing and tissue regeneration . Cell Tissue Res . 2007 ; 328 ( 3 ): 487 - 97 .

Singh AK , Patel J , Litbarg NO , Gudehithlu KP , Sethupathi P , Arruda JAL , et al . Stromal cells cultured from omentum express pluripotent markers, produce high amounts of VEGF, and engraft to injured sites . Cell Tissue Res . 2008 ; 332 ( 1 ): 81 - 8 .

Liebermann-Meffert D . The greater omentum. Anatomy, embryology, and surgical applications . Surg Clin North Am . 2000 ; 80 ( 1 ): 275 - 93 .

Vernik J , Singh AK . Omentum: Power to heal and regenerate . Int J Artif Organs . 2007 ; 30 ( 2 ): 95 - 9 .

Garcia-Gomez I , Pancholi N , Patel J , Gudehithlu KP , Sethupathi P , Hart P , et al . Activated omentum slows progression of CKD . J Am Soc Nephrol . 2014 ; 25 ( 6 ): 1270 - 81 .

di Nicola V . Omentum a powerful biological source in regenerative surgery . Regen Ther . 2019 ; 11 : 182 - 91 .

Lloris-Carsí JM , Barrios C , Prieto-Moure B , Lloris-Cejalvo JM , Cejalvo-Lapeña D . The effect of biological sealants and adhesive treatments on matrix metalloproteinase expression during renal injury healing . PLoS One . 2017 ; 12 ( 5 ): e0177665 .

Metro MJ , McAninch JW . Surgical exploration of the injured kidney: current indications and techniques . Int Braz J Urol . 2003 ; 29 ( 2 ): 98 - 105 .

Santucci RA , McAninch JW . Diagnosis and management of renal trauma: past, present, and future . J Am Coll Surg . 2000 ; 191 ( 4 ): 443 - 5 .

Delany HM , Ivatury RR , Blau SA , Gleeson M , Simon R , Stahl WM . Use of biodegradable (PGA) fabric for repair of solid organ injury: a combined institution experience . Injury . 1993 ; 24 ( 9 ): 585 - 9 .

Zhuang Q , Ma R , Yin Y , Lan T , Yu M , Ming Y . Mesenchymal stem cells in renal fibrosis: the flame of cytotherapy . Stem Cells Int . 2019 ; 2019 : 8387350 .

Zuk PA , Zhu M , Mizuno H , Huang J , Futrell JW , Katz AJ , et al . Multilineage cells from human adipose tissue: implications for cell-based therapies . Tissue Eng . 2001 ; 7 ( 2 ): 211 - 28 .

Charbord P . Bone marrow mesenchymal stem cells: historical overview and concepts . Hum Gene Ther . 2010 ; 21 ( 9 ): 1045 - 56 .

Tögel F , Weiss K , Yang Y , Hu Z , Zhang P , Westenfelder C . Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury . Am J Physiol Renal Physiol . 2007 ; 292 ( 5 ): F1626 - 35 .

Zhao JJ , Liu JL , Liu L , Jia HY . Protection of mesenchymal stem cells on acute kidney injury . Mol Med Rep . 2014 ; 9 ( 1 ): 91 - 6 .

Baer PC , Geiger H . Mesenchymal stem cell interactions with growth factors on kidney repair . Curr Opin Nephrol Hypertens . 2010 ; 19 ( 1 ): 1 - 6 .

Iwai S , Sakonju I , Okano S , Teratani T , Kasahara N , Yokote S , et al . Impact of ex vivo administration of mesenchymal stem cells on the function of kidney grafts from cardiac death donors in rat . Transplant Proc . 2014 ; 46 ( 5 ): 1578 - 84 .

Reinders MEJ , Fibbe WE , Rabelink TJ . Multipotent mesenchymal stromal cell therapy in renal disease and kidney transplantation . Nephrol Dial Transplant . 2010 ; 25 ( 1 ): 17 - 24 .

Mias C , Lairez O , Trouche E , Roncalli J , Calise D , Seguelas MH , et al . Mesenchymal stem cells promote matrix metalloproteinase secretion by cardiac fibroblasts and reduce cardiac ventricular fibrosis after myocardial infarction . Stem Cells . 2009 ; 27 ( 11 ): 2734 - 43 .

Khalid U , Pino-Chavez G , Nesargikar P , Jenkins RH , Bowen T , Fraser DJ , et al . Kidney ischaemia reperfusion injury in the rat: the EGTI scoring system as a valid and reliable tool for histological assessment . J Histol Histopathol . 2016 ; 3 : 1 - 7 .

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Role of the immune microenvironment in bone, cartilage, and soft tissue regeneration: from mechanism to therapeutic opportunity

Small molecules facilitate single factor-mediated sweat gland cell reprogramming

Efficient and rapid conversion of human astrocytes and ALS mouse model spinal cord astrocytes into motor neuron-like cells by defined small molecules

Communication Technology in Two-out-of-Two Computer Interlocking System

Simulation Analysis on Static Performance of EP Solenoid Valves Based on ANSYS

Related Author

Guo-Hui Liu

Shuan-Hu Zhou

Qian Feng

Anish Patel

Yuan Xiong

Bo-Bin Mi

Ze Lin

Yi-Qiang Hu

Related Institution

Harvard Stem Cell Institute, Harvard University

Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration

Department of Chemical and Biomolecular Engineering, Ohio University

Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University

⁰