Comparative study on the anti-tuberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan

Li-Yao Duan; Yan Liang; Wen-Ping Gong; Yong Xue; Jie Mi; Jie Wang; Lan Wang; Zai-Xing Jia; Hong Lei; Yu-Mei Liang; Jun Liu; Yue Zheng; Xue-Qiong Wu

doi:10.1186/s40779-021-00324-5

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Comparative study on the anti-tuberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan

RESEARCH | Updated：2023-02-28

- Comparative study on the anti-tuberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan
- Military Medical Research Vol. 9, Issue 2, Pages: 164-183(2022)
- Affiliations：
  
  1.Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Tuberculosis Research Institute, the 8th Medical Center, Chinese PLA General Hospital, Beijing 100091, China
  2.Hebei North University, Zhangjiakou 075000, Hebei, China
  3.Clinical Laboratory, the 8th Medical Center, Chinese PLA General Hospital, Beijing 100091, China
  4.Pathology Department, the 8th Medical Center, Chinese PLA General Hospital, Beijing 100091, China
  5.Guangdong Qifang Pharmaceutical Co., Ltd, Guangzhou 510075, China
- Author bio：
  
  * zhengy309@126.com;
  xueqiongwu@139.com
- Funds：
- DOI：10.1186/s40779-021-00324-5
  CLC：
- Published：2022-04
- Accepted：
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Duan et al.: Comparative study on the antituberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan. Mil Med Res, 2021, 8: 34.
DOI：

Duan et al.: Comparative study on the antituberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan. Mil Med Res, 2021, 8: 34. DOI： 10.1186/s40779-021-00324-5.

摘要

Abstract

Background:

The traditional Chinese medicine NiuBeiXiaoHe (NBXH) extract and Chinese medicine preparation JieHeWan (JHW) exhibit anti-tuberculosis (TB) effects. The anti-TB effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH.

Methods:

BALB/c mice aged 6-8 weeks were randomly divided into a normal control group

TB model group

JHW treatment group

and NBXH treatment group. After 3 and 13 weeks of treatment

the therapeutic effect in each group was evaluated by comparing lung histopathology

lung and liver colony counts

the number of spots representing effector T cells secreting IFN-γ in an enzyme-linked immunospot assay (ELISPOT)

and the levels of Th1

Th2

and Th17 cytokines

which were measured by a cytometric bead array (CBA). Mouse RNA samples were subjected to transcriptome sequencing.

Results:

After 13 weeks of treatment

the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group (

0.05). Compared with those in the TB model group

the lung colony counts in the JHW and NBXH groups were significantly decreased (

0.05)

and the IL-2 and IL-4 levels in the NBXH group were significantly increased (

0.05). NBXH partly restored significant changes in gene expression caused by

Mycobacterium tuberculosis

(

M. tuberculosis

) infection. According to GO and KEGG analyses

the changes in biological process (BP)

cell composition (CC) and molecular function (MF) terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent

but they were mainly related to the immune response and inflammatory response in the mouse TB model.

Conclusions:

NBXH had therapeutic effects similar to those of JHW in improving lung histopathology

reducing lung colony counts

and regulating the levels of cytokines. NBXH restored significant changes in gene expression and repaired cell damage caused by

M. tuberculosis

infection by regulating immune-related pathways

which clarified the mechanism of action of NBXH.

关键词

Keywords

references

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