Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Shuai Wei; Wei Wang; Li Li; Hao-Ye Meng; Chun-Zhen Feng; Yu-Ying Dong; Xi-Chi Fang; Qi-Qiang Dong; Wen Jiang; Hai-Li Xin; Zhan-Zhen Li; Xin Wang

doi:10.1186/s40779-021-00308-5

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Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

RESEARCH | Updated：2023-02-28

- Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs
- Military Medical Research Vol. 8, Issue 4, Pages: 510-522(2021)
- Affiliations：
  
  1.Institute of Orthopaedics, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Chinese PLA General Hospital, Beijing 100583, China
  2.Zhoushan Dinghai Guanghua Hospital, Zhoushan 316000, Zhejiang, China
  3.Tianjin Hospital, Tianjin University, Tianjin 300211, China
  4.Geriatric Neurological Department of the Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing 100853, China
  5.Department of Orthopedics, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, China
  6.Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, China
  7.Department of Plastic Surgery, General Hospital of Taiyuan Iron and Steel Limited Company, Taiyuan 030009, China
  8.Hand Microsurgery Department, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong, China
  9.Third Surgery Department, Zhengzhou Renji Hospital, Zhengzhou 450000, China
  10.Department of Orthopedics, the First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang 832000, China
  11.Pharmacy Department, Chinese PLA General Hospital, Beijing 100853, China
- Author bio：
  
  * lizz2688@163.com;
  wangx126@126.com
- Funds：
- DOI：10.1186/s40779-021-00308-5
  CLC：
- Published：2021-12
- Accepted：
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Wei et al.: Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs. Mil Med Res, 2021, 8: 18.
DOI：

Wei et al.: Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs. Mil Med Res, 2021, 8: 18. DOI： 10.1186/s40779-021-00308-5.

摘要

Abstract

Background:

Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.

Methods:

We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations (0

and 100 ng/ml) and different EGF action times (2

and 30 min). A full-thickness skin defect model was established using male

30-week-old Bama pigs. The experiment included groups as follows: routine dressing change after covering with sterile auxiliary material (Control)

continuous negative pressure drainage of the wound (VSD)

continuous negative pressure drainage of the wound and injection of EGF 10 min followed by removal by continuous lavage (V+E 10 min)

and continuous negative pressure drainage of the wound and injection of EGF 30 min followed by removal by continuous lavage (V+E 30 min). The wound healing rate

histological repair effect and collagen deposition were compared among the four groups.

Results:

An EGF concentration of 10 ng/ml and an action time of 10 min had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells. The drug dispersion effect was better than drug infusion after bolus injection effect

and the contact surface was wider. Compared with other groups

the V+E 10 min group promoted wound healing to the greatest extent and obtained the best histological score.

Conclusions:

A recombinant human epidermal growth factor (rhEGF) concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent

in vitro

. VSD combined with rhEGF kept in place for 10 min and then washed

can promote wound healing better than the other treatments

in vitro

关键词

Keywords

references

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