Characteristics of cytokines in the sciatic nerve stumps and DRG after rat sciatic nerve crush injury

Rui-Rui Zhang; Sai-Ling Chen; Zhang-Chun Cheng; Yin-Ying Shen; Sheng Yi; Hui Xu

doi:10.1186/s40779-020-00286-0

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Characteristics of cytokines in the sciatic nerve stumps and DRG after rat sciatic nerve crush injury

RESEARCH | Updated：2023-02-28

- Characteristics of cytokines in the sciatic nerve stumps and DRG after rat sciatic nerve crush injury
- Military Medical Research Vol. 8, Issue 3, Pages: 352-362(2021)
- Affiliations：
  
  1.Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu Province, China
  2.College of Medicine, Nantong University, Nantong 226001, Jiangsu Province, China
- Author bio：
  
  * syi@ntu.edu.cn;
  huixu82@126.com
- Funds：
- DOI：10.1186/s40779-020-00286-0
  CLC：
- Published：2021-09
- Accepted：
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Zhang et al.: Characteristics of cytokines in the sciatic nerve stumps and DRG after rat sciatic nerve crush injury. Mil Med Res, 2020, 7: 57.
DOI：

Zhang et al.: Characteristics of cytokines in the sciatic nerve stumps and DRG after rat sciatic nerve crush injury. Mil Med Res, 2020, 7: 57. DOI： 10.1186/s40779-020-00286-0.

摘要

Abstract

Background:

Cytokines are essential cellular modulators of various physiological and pathological activities

including peripheral nerve repair and regeneration. However

the molecular changes of these cellular mediators after peripheral nerve injury are still unclear. This study aimed to identify cytokines critical for the regenerative process of injured peripheral nerves.

Methods:

The sequencing data of the injured nerve stumps and the dorsal root ganglia (DRG) of Sprague-Dawley (SD) rats subjected to sciatic nerve (SN) crush injury were analyzed to determine the expression patterns of genes coding for cytokines. PCR was used to validate the accuracy of the sequencing data.

Results:

A total of 46

and 54 upstream cytokines were differentially expressed in the SN at 1 day

4 days

and 7 days after nerve injury. A total of 25

and 34 upstream cytokines were differentially expressed in the DRG at these time points. The expression patterns of some essential upstream cytokines are displayed in a heatmap and were validated by PCR. Bioinformatic analysis of these differentially expressed upstream cytokines after nerve injury demonstrated that inflammatory and immune responses were significantly involved.

Conclusions:

In summary

these findings provide an overview of the dynamic changes in cytokines in the SN and DRG at different time points after nerve crush injury in rats

elucidate the biological processes of differentially expressed cytokines

especially the important roles in inflammatory and immune responses after peripheral nerve injury

and thus might contribute to the identification of potential treatments for peripheral nerve repair and regeneration.

关键词

Keywords

references

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