Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and <sup>60</sup>Co γ-rays

Ding-Wen Jiang; Qing-Rong Wang; Xian-Rong Shen; Ying He; Tian-Tian Qian; Qiong Liu; Deng-Yong Hou; Yu-Ming Liu; Wei Chen; Xin Ren; Ke-Xian Li

doi:10.1186/s40779-017-0116-7

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Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and ⁶⁰Co γ-rays

RESEARCH | Updated：2026-03-12

- Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and ⁶⁰Co γ-rays
- Military Medical Research Vol. 4, Issue 3, Pages: 141-148(2017)
- Affiliations：
  
  The PLA Key Laboratory of Biological Effect and Medical Protection on Naval Vessel Special Environment, Naval Medical Research Institute, Xiangyin Road 880, Shanghai 200433, China
- Author bio：
  
  *: xianrong_sh@163.com
- Funds：
- DOI：10.1186/s40779-017-0116-7
  CLC：
- Published：2017-09
- Accepted：
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Jiang et al.: Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and ⁶⁰Co γ-rays. Mil Med Res, 2017, 4: 7
DOI：

Jiang et al.: Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and ⁶⁰Co γ-rays. Mil Med Res, 2017, 4: 7 DOI： 10.1186/s40779-017-0116-7.

摘要

Abstract

Background:

Cimetidine

an antagonist of histamine type II receptors

has shown protective effects against γ-rays or neutrons. However

there have been no reports on the effects of cimetidine against neutrons combined with γ-rays. This study was carried out to evaluate the protective effects of cimetidine on rats exposed to long-term

low-dose-rate neutron and γ-ray combined irradiation (n-γ LDR).

Methods:

Fifty male Sprague-Dawley (SD) rats were randomly divided into 5 groups: the normal control group

radiation model group

20mg/(kg·d) cimetidine group

80mg/(kg·d) cimetidine group and 160mg/(kg·d) cimetidine group (10 rats per group). Except for the normal control group

40 rats were simultaneously exposed to fission neutrons (

252

0.085mGy/h) for 22h every day and γ-rays (

0.097Gy/h) for 1.03h once every three days

and the cimetidine groups were administered intragastrically with cimetidine at doses of 20

80 and 160mg/kg each day. Peripheral blood WBC of the rats was counted the day following exposure to γ-rays. The rats were anesthetized and sacrificed on the day following exposure to

252

Cf for 28 days. The spleen

thymus

testicle

liver and intestinal tract indexes were evaluated. The DNA content of bone marrow cells and concanavalin A (ConA)-induced lymphocyte proliferation were measured. The frequency of micronuclei in polychromatic erythrocytes (fMNPCEs)

superoxide dismutase (SOD)

malondialdehyde (MDA)

and glutathione peroxidase (GSH-Px) in the serum and liver tissues were detected.

Results:

The peripheral blood WBC in the cimetidine groups was increased significantly on the 8th day and the 26th day compared with those in the radiation model group. The spleen

thymus and testicle indexes of the cimetidine groups were higher than those of the radiation model group. The DNA content of bone marrow cells and lymphocyte proliferation in the cimetidine groups were increased significantly

and fMNPCE was reduced 1.41-1.77 fold in cimetidine treated groups. The activities of SOD and GSH-Px in the cimetidine groups were increased significantly

and the content of MDA in the cimetidine groups was decreased significantly.

Conclusions:

The results suggested that cimetidine alleviated damage induced by long-term

low-dose-rate neutron and γ combined irradiation via antioxidation and immunomodulation. Cimetidine might be useful as a potent radioprotector for radiotherapy patients as well as for occupational exposure workers.

关键词

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references

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Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and 60Co γ-rays

DOI：10.1186/s40779-017-0116-7

摘要

Abstract

关键词

Keywords

references

Radioprotective effects of cimetidine on rats irradiated by long-term, low-dose-rate neutrons and ⁶⁰Co γ-rays