Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity

张忠兵; 张学庸; 陈希陶

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Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity

Updated：2026-03-12

- Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity
- Military Medical Research Issue 2, Pages: 159-164(1986)
- Affiliations：
  
  1. Department of Gastroenterology The First Affiliated Hospital
  2. The Fourth Military Medical College
  3. Xian
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：1986
- Accepted：
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[1]张忠兵,张学庸,陈希陶.Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity[J].Journal of Medical Colleges of PLA,1986(02):159-164.
DOI：

[1]张忠兵,张学庸,陈希陶.Practical Value of Identification of T-Lymphocytes in Paraffin Embeded Gastroduodenal Tissues and Perigastric Lymph Nodes by Demonstration of Acid α-Naphthyl Acetate Esterase Activity[J].Journal of Medical Colleges of PLA,1986(02):159-164. DOI：

摘要

Abstract

<正> Acid α-naphthyl acetate esterase（ANAE）activity was used todemonstrate T lymphocytes in paraffin-embedded gastrointestinal tissuesand perigastric lymph nodes

one or more reddish-brown dots could berecognized in the cytoplasm of T lymphocytes

diffuse reddish-brownANAE products were also present in the cytoplasm of macrophages

epithelial and glandular cells.B lymphocytes were ANAE negative.Itwas demonstrated that ANAE cytochemical staining technique could beused to differentiate T lymphocytes.This method is simple and easy tocarry out in most hospitals.The percentage of T lymphocytes in essentially normal gastric mucosawas about 9.8％ There was no significant difference in fundi andantra.The intraepithelial cells were located at the bottom of epithe-lial cells.In lamina propria T lymphocytes were distributed near the ba-sal layer of the epithelium.This might be related to the perception ofantigenic stimulation and regulation of immunologic reaction.

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