Method for Ultrastructural Localization of N-acetylcholine Receptor

陈志; 郑尊; 蒋鲁丽; 杨雨善

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Method for Ultrastructural Localization of N-acetylcholine Receptor

Updated：2026-03-12

- Method for Ultrastructural Localization of N-acetylcholine Receptor
- Military Medical Research Issue 2, Pages: 130-134(1986)
- Affiliations：
  
  1. Department of Biochemistry and Central Laboratory Second Military Medical College
  2. ,Shanghai
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：1986
- Accepted：
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[1]陈志,郑尊,蒋鲁丽,杨雨善.Method for Ultrastructural Localization of N-acetylcholine Receptor[J].Journal of Medical Colleges of PLA,1986(02):130-134.
DOI：

[1]陈志,郑尊,蒋鲁丽,杨雨善.Method for Ultrastructural Localization of N-acetylcholine Receptor[J].Journal of Medical Colleges of PLA,1986(02):130-134. DOI：

摘要

Abstract

正

A method to localize N-acetylcholine receptor at neuromuscular junctionby using α-BT-HRP conjugate is described.Intact strips of fresh musclewere incubated with α-BT-HRP conjugate at a concentration of 1×10

-7

Min Tyrode’s solution and then reacted with Karnovsky’s DAB medium.Various vesicular forms of brownish red reaction products were seen un-der light microscope.Under electron microscope

reaction products wereseen both on the presynaptic and postsynaptic membranes.The bindingactivity and reliability of α-BT-HRP are discussed.

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