Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains

马文煜; 姜绍谆; 于碧云; 汪美先; 李学荣; 魏正乾

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Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains

Updated：2026-03-12

- Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains
- Military Medical Research Issue 4, Pages: 356-362(1986)
- Affiliations：
  
  1. Department of Microbiology Fourth Military Medical College
  2. ,Xi’an
  3. Department of Electron Microscope
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：1986
- Accepted：
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[1]马文煜,姜绍谆,于碧云,汪美先,李学荣,魏正乾.Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains[J].Journal of Medical Colleges of PLA,1986(04):356-362.
DOI：

[1]马文煜,姜绍谆,于碧云,汪美先,李学荣,魏正乾.Concentration and Purification of Japanese Encephalitis Virus from Infected Mouse Brains[J].Journal of Medical Colleges of PLA,1986(04):356-362. DOI：

摘要

Abstract

<正> A suspension was prepared from the infant mouse brain in-fected by JEV.It was clarified by centrifugation.Protamine sulfatewas added to the supernatant with a final concentration of 5 mg/ml

and the contaminated protein was removed.The recovery of HAand infectivity was 66% and 55% respectively.The supernatant was concentrated by differential centrifugationand purified by rate zone centrifugation in sucrose density gradientwith recovery of 29% of HA and 37% infectivity.The purificationfactors（PF）were 138 for HA and 175 for infectivity.SHA without any infectivity were found on the top of gradientcolumn.The immunological characteristics of the componentsawait further studies.

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