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Interferon-gamma（IFN-γ）was induced by pokeweed mitogen（PWM）or esculentoside in human splenic lymphocytes.PWM-inducedIFN-γ was partially purified to a specific activity of 10

6

RU/mgprotein by batch adsorption on controlled-pore glass（CPG）beadsand desorption by ethylene glycol in phosphate-buffered saline（PBS）.Use of Chinese made micro-pore glass（MPG）instead of CPG topurify IFN-γ showed similar purification efficacy.Both CPG andMPG recovered could be regenerated for repeated use.The yieldfrom glass beads was further purified to a specific activity of 10

7

RU/mg protein

with about 78% recovery

by the anti-new-borncalf serum antibodies-Sepharose 4B（ACS）column chromatography.Almost all the antiviral activity of the IFN-γ induced by PWM oresculentoside was destroyed by adjusting to pH 2 or heating to 56℃

and was neutralized by monoclonal antibody to human interferon-γ（Hu IFN-γ）

thereby the product was identified as Hu IFN-γ.Bio-gel P-200 chromatography resulted in two peaks of activity for PWM-induced IFN

a major one with molecular weight（MW）of 40

000-45

700 and a minor one of 25

700

but only one peak of 45

700-49

000for esculentoside-induced IFN.