Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering

杨静华; 项秉懿; 陈南春; 陈苏民

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Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering

Updated：2026-03-12

- Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering
- Military Medical Research Issue 2, Pages: 181-185(1989)
- Affiliations：
  
  1. Department of Biochemistry Fourth Military Medical University
  2. ,Xi’an
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：1989
- Accepted：
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[1]杨静华,项秉懿,陈南春,陈苏民.Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering[J].Journal of Medical Colleges of PLA,1989(02):181-185+187.
DOI：

[1]杨静华,项秉懿,陈南春,陈苏民.Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering[J].Journal of Medical Colleges of PLA,1989(02):181-185+187. DOI：

摘要

Abstract

<正> Eight oligonucleotide fragments were designed with the aid of a computer and synthesizedaccording to the amino add sequcnce of human atrial natriuretic factor（ANF）.By means of an-nealing and ligation

these fragments were assembled into an overlapping concatenator consisting oftwo ANF genes ligated by TGATG for termination and initiation of translation.Theconcatenator was omserted into plasmid pRC23 and the recobinant DNA was transformed into E.coli strain TAP106.Analysis by restriction enzyme mapping

hybridization and DNA sequenongshowed that the orientation and reading frame of the gene were correct.

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