<正> In this study

the DNA probe pPF14 was nonradioactively labelled by sulfo-modifica-tion

and used in a dot blot hybridization assay for detection of P.falciparum.The resultsshowed that the sulfoprobe successfully detected 25pg purified DNA and 0.001% parasitemia ofcultured P.falciparum

and did not react with human leukocyte DNA.In the study of 179clinical blood samples of malaria patients from Yunnan province

the DNA probe results corre-lated well with blood smear ones.Of 107 P.falciparum samples determined by microscope ex-amination

99 were positive by sulfoprobe （92.5%）;Of 72 P.vivax samples

1 was crosslyreacted;no cross reactions were found with any of 48 normal blood samples.It is indicated thatsulfoprobe may be useful in specific diagnosis and epidemiological investigation of P.falciparuminfection.