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The effects of combined use of earthworm extract（912）and HpD-laser on the produc-tion of reactive oxygen and the biosynthesis of DNA in S

180

tumor cells were studied throughchemiluminescence measurement and[

3

H]-TdR incorporation assay.The results showed that as com-pared with the control

the intensity of chemiluminescence emitted by tumor cells treatedsimultaneously with 912 and HpD-laser was enhanced more than ten-folds

while that treated with912 or HpD-laser alone was increased only 2～4 folds.The[

3

H]-TdR incorporation into tumorcells of the former group was inhibited upto 74.1%

and that of the latter groups decreased onlyby 42.2% and 40.0%

respectively.In accordance with these biochemical changes

the ultrastructuraldamage of tumor cells of the former

combinedly treated group appeared to be the most serious.This suggests an additive effect of 912 with HpD-laser on tumor cells.In addition

if free radicalscavengers

such as catalase and superoxide dismutase

were added to the reaction systembefore chemiluminescence assay

the luminescent enhancement effect mentioned above was dramaticallyalleviated

implying the presence of O

2

and H

2

O

2

in the system.Therefore

as to the toxic effecton tumor cells

912 and HpD-laser are not only additive in efficiency

but also similar in theunderlying mechanism of action.