<正>Objective:To observe the differential expression of TGF-β-induced gene human clone 3 （βig-h3） in human hepatoma cell lines. Methods: Human hepatoma cells HHCC. 7721. T7721 constructed by stably transfecting HAbl8G/CD147 cDNA into 7721 and normal human liver cell QZG were cultured as previously. RT-PCR and western blot were used to investigate the differential expression ofβig-h3 in human hepatoma cell lines and normal liver cell. Results:The results of RT-PCR suggested that the expression ofβig-h3 mRNA in human hepatoma cells was higher than that in normal human liver cell QZG（P< 0. 01）

and its expression level in human hepatoma cells in turn was HHCC>T7721>7721. Moreover

the similar results ofβig-h.3 protein expression were testified by western blot. Conclusion: This study demonstrates that the expression ofβig-h3 in hepatoma cell lines is higher than that in normal liver cell QZG

which provides a sound basis for exploring the function ofβig-h3 in processes of adhesion and metastasis of human hepatoma cells.