<正>Objective: To investigate the chemical constituents of Polygala japonica Houtt. . Methods: The constituents were separated and purified by column chromatographies with silical gel

RP-silical gel and Sephadex LH-20. Their structures were elucidated on the basis of spectral data （IR

MS

1H-NMR

13 C-NMR

HMQC and HMBC）. Results: Totally 15 compounds were obtained from Polygala japonica Houtt. and their structures were identified as castaprenol-12 （ I ）

β-sitosterol （ II ）

stigmasterol （ I ）

stigmasta-7

22-di-ene-3-one （IV ）

n-dotriacontanol （V ）

n-hexadecanol （VI）

arachidic acid （ VI ）

ero-gosterol （ VIIII ）

kaempferol （ IX ）

quercetin （ X ）

daucosterol （ XI ）

p-hydroxybenzoic acid （ XI ）

coumaric acid （ XIII ）

rhamnocitrin 3-O-β-D-galactopyranoside （ XIV ）

quercetin 3-O-β-D-glucopyranoside （ XV ）. Conclusion: Compounds I

I

IV

V

VI

VII

VIII

XII

XIII and XIV were obtained from this plant for the first time.