<正>Objective:To evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide （AS-ODN） on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. Methods:The AS-ODN complementary to the start codon region of heparanase mRNA and its control

scrambled nonsense oligodeoxynucleotide （NS-ODN） were designed and synthesized.A subcutaneous growth model and an acute hematogenous metastasis model of human mammary carcinoma were estab- lished in nude mice and were treated with ODNs.The heparanase expression in tumor was evaluated by RT-PCR and Western blot.The microvessel density （MVD） was measured by immunohistochemistry for factorⅧ.The tumor volume was calculated and lung metastatic nodules were counted.Results:The hep- aranase expression

MVD

tumor volume and lung metastatic nodules in AS-ODN treated group were sig- nificantly decreased compared with that in NS-ODN treated group and that in PBS group （P＜0.01）.Con- clusion:Heparanase AS-ODN has significant inhibitory effect on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice.