<正>Objective:Duchenne muscular dystrophy（DMI）) and Becker muscular dystrophy（BMD） are X-linked disorders caused by mutations in the dystrophin gene.The majority of recognized mutations are copy number changes of individual exons.The objective of the present study was to assess the multiplex [igation dependent probe amplification（MLPA） effects of detection of gent mutations.Methods:Samples of 2o control males and 80 males and their mothers referred to our diagnostic facility on the clinical suspi- cion of DMD or BMD were tested by MI

PA and multiplex PCR.Results:The mean DQs for all peak of 20 control male samples was I

02 （range from 0.83to 1.21） by MLPA.Deletions or duplications wereiden tified in 6 out of 31 families that had been previously tested as negative by multiplex PCR.One case of complex rearrangement involving a duplication of two regions:dupEX3-9 and dupEX 17-41 were found by MLPA.Conclusions:MLPA is a highly sensitive method and rapid alternative to multiplex PCR for detec tion of DMD and BMI).