Construction of eukaryotic expression vector encoding ATP synthase lipid-binding protein-like protein gene of Sj and its expression in HeLa cells

Ouyang Danming Li Mulan Yuan Caijia Faculty of Laboratory Medicine; Xiangnan University; Chenzhou 423000; China Hu Yongxuan Department of Microbiology and Immunology; Xiangnan University; Chenzhou 423000; China Zeng Xiaojun Chenzhou Center of Disease Control and Prevention; Chenzhou 423000; China He Zhixiong First People’s Hospital ofChenzhou; Chenzhou 423000; China

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Construction of eukaryotic expression vector encoding ATP synthase lipid-binding protein-like protein gene of Sj and its expression in HeLa cells

Updated：2026-03-12

- Construction of eukaryotic expression vector encoding ATP synthase lipid-binding protein-like protein gene of Sj and its expression in HeLa cells
- Military Medical Research Issue 2, Pages: 94-97(2008)
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  CLC： Q78
- Published：2008
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[1].Construction of eukaryotic expression vector encoding ATP synthase lipid-binding protein-like protein gene of Sj and its expression in HeLa cells[J].Journal of Medical Colleges of PLA,2008(02):94-97.
DOI：

[1].Construction of eukaryotic expression vector encoding ATP synthase lipid-binding protein-like protein gene of Sj and its expression in HeLa cells[J].Journal of Medical Colleges of PLA,2008(02):94-97. DOI：

摘要

Abstract

正

Objective:To clone and construct the recombinant plasmid containing ATP synthase lipid-binding protein-like protein gene of Schistosoma japonicum

（SjAslp） and transfer it into mammalian cells to express the objective protein. Methods:By polymerase chain reaction （PCR） technique

SjAslp was amplified from the constructed recombinant plasmid pBCSK

/SjAslp

and inserted into cloning vector pUCm-T.Then

SjAslp was subcloned into an eukaryotic expression vector pcDNA3.1（+）.After identifying it by PCR

restrictive enzymes digestion and DNA sequencing

the recombinant plasmid was transfected into HeLa cells using electroporation

and the expression of the recombinant protein was analyzed by immunocytochemical assay.Results:The specific gene fragment of 558 bp was successfully amplified.The DNA vaccine of SjAslp was successfully constructed.Immunocytochemical assay showed that SjAslp was expressed in the cytoplasm of HeLa cells.Conclusion:SjAslp gene can be expressed in eukaryotic system

which lays the foundation for development of the SjAslp DNA vaccine against schitosomiasis.

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