Objective:To explore the construction of metastatic spinal cancer（MSC） tissue microarrays and validate its value in immunohistochemical study of MSC.Methods:Paraffin-embedded specimens from 71 MSC cases and 6 primary tumor cases were selected as donor blocks and prepared into MSC tissue microarrays by tissue array arrangement

the steps of which included location

punching

sampling

sample seeding

and re-diagnosis by hematoxylin-eosin（HE） as well as MMP-9 and MMP-14 immunohistochemical staining.Results:The MSC tissue microarrays thus constructed were intact and crackless

containing 154 complete and well arranged microarray points.None of the sectioned tissue microarrays was lost

and the results of HE staining was consistent with the primary pathologic diagnoses.Immunohistochemical staining was also good without non-specific or marginal effect.Conclusion:The MSC tissue microarrays have a high value in the immunohistochemical study of MSC.