Objective: To investigate the feasibility of intra-operative radiation therapy （IORT） in hepato-cellular carcinoma （HCC）. Methods: Based on the dosage distribution of tumor and adjacent sensitive organs

and dose-volume histogram （DVH）

Topslane three-dimensional therapy plan was used to compare IORT and three-dimensional conformal radiation therapy （3D-CRT） in 12 cases of HCC. Results: Taking the center of tumors as the isodose center

the V90 （volume of 90% dose distribution） of IORT was significantly better than that of 3D-CRT

and median absorbed doses of normal organs in IORT was significantly lower than that in 3D-CRT. Conclusion: The V90 of IORT is better than that of 3D-CRT in HCC

and neighboring sensitive organs were effectively protected by IORT. The tumors absorbed dose and local control rate are improved in IORT.