Objective:To investigate the actions of cytokine profile in the immune cells by a seven amino acid peptide mimic from HVR1 of HCV （GQTYTSG

named 7P）.Methods:The peripheral blood mononuclear cells （PBMCs） from 17 healthy blood donors were stimulated with 7P

and the cytokine levels in CD4+CD8-

CD4-CD8+ T cells

NK

NKT cells were analyzed by the intracellular cytokine staining.Results:The frequency of cells which secreted interferon-γ （IFN-γ） was found to be significantly increased in all cells

interleukin-10（IL-10） was significantly increased in CD4+CD8-

CD4-CD8+ T cells but decreased in NK

NKT cells

and tumor necrosis factor-α （TNF-α） was decreased in CD4+CD8-

CD4-CD8+ but increased in NK cells.Conclusion:7P could induce a cytokine profile in different immune cells in vitro and there was some difference between the CD4+CD8-

CD4-CD8+ T cells which represented the adaptive immunity cells and NK

NKT cells which represented the innate immunity cells.This kind of variation of cytokine profile might contribute to anti-virus and anti-inflammatory immune reaction.