A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing

Chun-Xiao Li; Hai-Li Qian

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A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing

COMMENTARY | Updated：2022-12-27

- A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing
- Military Medical Research Vol. 2, Issue 3, Pages: 121-122(2015)
- Affiliations：
  
  State Key Laboratory of Molecular Oncology, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing 200021, China
- Author bio：
  
  *: qianhaili001@163.com
- Funds：
- DOI：
  CLC：
- Published：2015-09
- Accepted：
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Chun-Xiao Li, Hai-Li Qian. A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing[J]. Military Medical Research, 2015, 2(3): 121-122.
DOI：

Chun-Xiao Li, Hai-Li Qian. A double-edged sword: CRISPR-Cas9 is emerging as a revolutionary technique for genome editing[J]. Military Medical Research, 2015, 2(3): 121-122. DOI：

摘要

Abstract

In May 2015

professor Xiao Yang authored a review on the development of CRISPR-Cas9 techniques in the journal of

Military Medical Research

. This review provided a valuable overview of this major scientific advance. It has been four years since the first publication of the CRISPR-Cas9 breakthrough. The use of this technique has expanded into various scientific areas and is being developed into a systematic technical platform that may contribute to many bioengineering fields involving DNA sequence editing.

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references

Yang X . Applications of CRISPR-Cas9 mediated genome engineering . Mil Med Res . 2015 ; 2 : 11 .

Jinek M , Chylinski K , Fonfara I , Hauer M , Doudna JA , Charpentier E . A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity . Science . 2012 ; 337 : 816 - 21 .

Doudna JA , Charpentier E . Genome editing. The new frontier of genome engineering with CRISPR-Cas9 . Science . 2014 ; 346 : 1258096 .

Ran FA , Hsu PD , Wright J , Agarwala V , Scott DA , Zhang F . Genome engineering using the CRISPR-Cas9 system . Nat Protoc . 2013 ; 8 : 2281 - 308 .

Shalem O , Sanjana NE , Hartenian E , Shi X , Scott DA , Mikkelsen TS , et al . Genome-scale CRISPR-Cas9 knockout screening in human cells . Science . 2014 ; 343 : 84 - 7 .

Guo Y , Xu Q , Canzio D , Shou J , Li J , Gorkin DU , et al . CRISPR Inversion of CTCF Sites Alters Genome Topology and Enhancer/Promoter Function . Cell . 2015 ; 162 : 900 - 10 .

Kleinstiver BP , Prew MS , Tsai SQ , Topkar VV , Nguyen NT , Zheng Z , et al . Engineered CRISPR-Cas9 nucleases with altered PAM specificities . Nature . 2015 ; 523 : 481 - 5 .

Samai P , Pyenson N , Jiang W , Goldberg GW , Hatoum-Aslan A , Marraffini LA . Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas Immunity . Cell . 2015 ; 161 : 1164 - 74 .

Maruyama T , Dougan SK , Truttmann MC , Bilate AM , Ingram JR , Ploegh HL . Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining . Nat Biotechnol . 2015 ; 33 : 538 - 42 .

Kim D , Bae S , Park J , Kim E , Kim S , Yu HR , et al . Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells . Nat Methods . 2015 ; 12 : 237 - 43 .

Liang P , Xu Y , Zhang X , Ding C , Huang R , Zhang Z , et al . CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes . Protein Cell . 2015 ; 6 : 363 - 72 .

Lanphier E , Urnov F , Haecker SE , Werner M , Smolenski J . Don't edit the human germ line . Nature . 2015 ; 519 : 410 - 1 .

Cyranoski D , Reardon S . Embryo editing sparks epic debate . Nature . 2015 ; 520 : 593 - 4 .

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